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Peptides Academy

How to Reconstitute a Lyophilized Peptide

Reconstitution is the process of dissolving a lyophilized (freeze-dried) peptide powder in sterile bacteriostatic water (BAC) for injectable use. Done carefully, it preserves peptide integrity; done roughly, it can degrade the product.

How It Works

1) Allow both the peptide vial and BAC water to reach room temperature. 2) Wipe both rubber stoppers with alcohol. 3) Draw the desired volume of BAC water into an insulin syringe. 4) Slowly inject the water against the inside wall of the peptide vial — never direct the stream onto the powder, which can denature fragile peptide chains. 5) Do not shake. Gently swirl or let sit until fully dissolved. 6) Label the reconstituted vial with date and final concentration.

Benefits

Preserves peptide integrity vs forceful mixing
Establishes precise dose-per-unit concentration for syringe calculation
Extends shelf life by using bacteriostatic (not sterile) water

Recommended Products (2)

Frequently Asked Questions

BAC water vs sterile water — does it matter?
Bacteriostatic water contains 0.9% benzyl alcohol, which inhibits bacterial growth and enables 28-day use of a multi-dose vial. Sterile water is for single-dose use only.

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