Peptides AcademyHow to Reconstitute a Peptide
Reconstitution is the single highest-leverage technical skill in peptide self-administration. Done right, it preserves peptide integrity and gives you precise per-unit dosing. Here's the six-step standard protocol.
Warm both vials
Remove the lyophilized peptide and the BAC water from refrigeration 10–15 minutes before starting. Room-temperature liquids reduce thermal shock on fragile peptide chains.
Sanitize the stoppers
Swab both rubber stoppers with a fresh 70% isopropyl wipe. Any injection tool touching the stoppers afterwards should be sterile.
Choose diluent volume
Round numbers make dose math easier. For a 5 mg vial, 2 mL gives 2.5 mg/mL; 2.5 mL gives 2 mg/mL. More water = larger draw volume per dose.
Inject against the wall
Slowly depress the plunger so water runs down the inside wall of the peptide vial. Never direct the stream onto the powder — it denatures delicate peptide bonds.
Swirl to dissolve
Gently rotate the vial in slow circles for 30–60 seconds. Do not shake. Fully dissolved peptide is clear, colorless (except GHK-Cu, which is blue).
Label and store
Write the reconstitution date and concentration on the vial. Store at 2–8 °C. Most reconstituted peptides are stable 21–30 days. For longer storage, aliquot and freeze.
Calculate your dose
Now that the peptide is reconstituted, convert your target dose (in mg or mcg) into units on a U-100 insulin syringe — in one click.